Roles of NF-κB in expression of hBD-2 mRNA in human primary epithelial cells induced by TNF-α

Objective To explore the roles of NF-κB in expression of human β-defensin-2(hBD-2) mRNA induced by TNF-α in the human airway primary epithelial cells.Methods After the human bronchial primary epithelial cells were stimulated with TNFα or first with NF-κB inhibitor PDTC,then TNF-α,the expression of hBD-2 mRNA was detected by RT-PCR.The IκB-α protein level in the cytoplasm was detected by Western bl…