Shotgun Library Construction for DNA Sequencing

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Shotgun Library Construction for DNA Sequencing

Book-chapter 2004 EN

Shotgun cloning is a method to generate the templates needed for DNA sequencing. This process entails breaking a large target DNA randomly into smaller fragments; end sequencing these smaller fragments; and from the overlapping sequences of the randomly generated fragments, reassembling the initial target sequence. Although this random strategy initially was described more than two decades ago ( 1…

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1

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1 Bruce A. Roe Corresponding University of Oklahoma (US) —

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