Insertion of Membrane Proteins into Discoidal Membranes Usin…
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- DOI 10.1021/pr800265f
- PMID https://pubmed.ncbi.nlm.nih.gov/18557639
- OpenAlex ID W2007166888
- Type Article
- Language EN
- Published 17 Jun 2008
- Volume 7 (8)
- Pages 3535–3542
- Source Journal of Proteome Research
- Publisher American Chemical Society
- FWCI 4.09
- Countries 1
- Institutions 1
- Added 10 Oct 2025
- Updated 16 Oct 2026
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Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach
Article 2008 EN
We report a cell-free approach for expressing and inserting integral membrane proteins into water-soluble particles composed of discoidal apolipoprotein-lipid bilayers. Proteins are inserted into the particles, circumventing the need of extracting and reconstituting the product into membrane vesicles. Moreover, the planar nature of the membrane support makes the protein freely accessible from both…
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12 authors| # | Author | Institutions | ORCID |
|---|---|---|---|
| 1 | Federico Katzen | Lawrence Livermore National La… (US) | — |
| 2 | Julia E. Fletcher | Lawrence Livermore National La… (US) | — |
| 3 | Jian-Ping Yang | Lawrence Livermore National La… (US) | — |
| 4 | Douglas Kang | Lawrence Livermore National La… (US) | — |
| 5 | Todd C. Peterson | Lawrence Livermore National La… (US) | — |
| 6 | Jenny A. Cappuccio | Lawrence Livermore National La… (US) | — |
| 7 | Craig Blanchette | Lawrence Livermore National La… (US) | |
| 8 | Todd Sulchek | Lawrence Livermore National La… (US) | |
| 9 | Brett A. Chromy | Lawrence Livermore National La… (US) | — |
| 10 | Paul D. Hoeprich | Lawrence Livermore National La… (US) | — |
| 11 | Matthew A. Coleman | Lawrence Livermore National La… (US) | |
| 12 | Wieslaw A. Kudlicki Corresponding | Lawrence Livermore National La… (US) | — |
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