Proteome analysis of rat hippocampal neurons by multiple lar…

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Proteome analysis of rat hippocampal neurons by multiple large gel two-dimensional electrophoresis

Article 2002 EN

The goal of the present study was to detect as many protein spots as possible in mammalian cells using two-dimensional gel electrophoresis (2-DE). For proteome analysis, it is of importance to reveal as many proteins as possible. A single standard 2-DE gel (pH 3-10, 18 cm x 20 cm, 13.5% gel) could detect 853 spots from proteins of cultured rat hippocampal neurons when visualized by silver staining…

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1 Takashi Oguri Nara Institute of Science and … (JP) —
2 Iku Takahata Nara Institute of Science and … (JP) —
3 Kazuhiro Katsuta Nara Institute of Science and … (JP) —
4 Eiko Nomura Nara Institute of Science and … (JP) —
5 Masahiro Hidaka Nara Institute of Science and … (JP) —
6 Naoyuki Inagaki Corresponding Japan Science and Technology A… (JP) Nara Institute of Science and … (JP)

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