Physics-informed multi-encoder adaptive optics enables rapid aberration correction for intravital microscopy of deep complex tissue

Tissue-induced optical aberrations fundamentally constrain intravital microscopy in deep or complex biological specimens. While adaptive optics (AO) can compensate for these aberrations, conventional AO methods are limited by either guide-star dependency or slow correction speeds. Here, we develop MeNet-AO, a multi-encoder network-based AO method that enables rapid, guide-star-free aberration corr…