Preparation and Characterization of Monoclonal Antibody Against Human Malignant Lymphoma

BALB/C mice was immunized with Raji cell suspension.The cells were fused as a normal method and the MAb was screened by indirect ELISA. A murine MAb SZ138 was obtained and its titer in ascites was 1:3.2×10 4. MAb was typed by reaction with subclass-specific rabbit anti-mouse antibody and was found to be IgG 1. Flow cytometry showed that MAb SZ138 was postive with Raji and Daudi cells, while did no…