Preparation of monoclonal antibody against human tumor endothelial marker-7 extracellular fragment and study of the localization of its protein in carcinoma of different origins

Objective To prepare monoclonal antibody specifically against human tumor endothelial marker-7(TEM7), then to examine the localization of TEM7 protein in solid tumors and related normal tissues of different origins by immunohistochemical staining in order to provide a valuable theoretical basis for TEM7-based vascular targeting cancer therapy. Methods TEM7 extracellular cDNA fragment was cloned wi…