Effects of cryopreservation on different maturation stage of mouse oocytes

Objective: To evaluate the effects of vitrification on the rates of survival,in vitro maturation (IVM),development and cytoskeleton of mouse frozen-thawed oocytes at different stages in order to find the most suitable cryopreservation protocol. Methods: MⅡ,GV and IVM-MⅡ were vitrified. After thawed, they underwent in vitro maturation, in vitro fertilization,embryo cultivation or fixed for immunofl…