Establishment and verification of the mouse model of pulmonary fibrosis induced by bleomycin

Objective To investigate the change of molecular biology and histopalhology in the different periods of pulmonary fibrosis model induced by instillation of bleomycin(BLM).Methods Lung fibrosis was induced by a single intratracheal instillation of bleomycin(2.5 mg/kg) in C57BL/6 female mice of 6 to 8 weeks.To judge the development of pulmonary fibrosis of the mouse model,lung samples were collected…