The observation of skin explants culture to harvest primary cell

Objective To harvest primary cells from skin explants culture. Methods Skin explants in size of 2mm×2mm were obtained by using sharp scissors,each skin explant placed onto the bottom of culture dish,and they were oriented with the epidermis facing up,then observe the cells migrating from the explants. Results From the third day,keratinocytes migrating from the explants,then melanocytes appear arou…