Study on Cryopreservation of Mouse 2-Cell Embryos by Straw or OPS Vitrification

The results of cryopreservation of mouse 2-cell embryos by straw and OPS vitrification method in this study showed that when the 2-cell embryos were equilibrated in EFS40 at 20℃ for 1 min (one-step straw method) before cryopreservation, the blastocysts rate was only 35.0%. There was a significant difference (P0.01) from the control ((65.0%)). When 2-cell embryos were pretreated in 10% EG+10% D for…