Testosterone inhibition of LRH-induced luteinizing hormone release by cultures of rat anterior pituitary cells: effect of inhibitors of steroid 5α reductase

Cultures of rat anterior pituitary cells were utilized to examine the role of 5 alpha reduction in the inhibitory action of testosterone on LRH-induced LH release. Cultures were pre-treated with steroids for 48 h and then incubated 6 h with steroids plus 5 x 10(-10) M LRH, the approximate EC50 for LH and FSH release. Testosterone inhibited LRH-induced LH release with an IC50 of approximately 2 x 1…