Effect of Ethylene Glycol Concentration and Exposure Period of Vitrification on Morphological and Viability of Immature Cow Oocyte

The objective of this research was to find out the right time of exposure procedure in vitrification method towards morphology and viability of bovine oocytes. Aspirated oocytes were washed by PBS, then transferred into ethylene glikol cryoprotectant at 20%, 30%, 40%, 50% concentrations. Each concentration was enriched by adding 0,5 M sucrose, and the oocytes were further transferred in 0,25 cc mi…